Experimental Roadmap
A staged MVP plan intended for rigorous validation or falsification.
MVP summary
- MVP-0: sensor + hysteresis behavior (reporter assays; reversible control).
- MVP-1: mitochondrial targeting + response under redox/ETC stress (neuronal lines).
- MVP-2: functional neuroprotection (human iPSC neurons; network activity/synapses).
- MVP-3: adaptive mitophagy and energetic economy (mt-Keima, ATP biosensors).
- MVP-4: safety, specificity, shutdown fidelity (omics + kill-switch validation).
Core success criteria
- Demonstrate hysteresis and reversibility (avoid chatter).
- Normalize redox/respiration metrics under insult without basal hyperactivation.
- Preserve neuronal functional readouts in advanced models.
- Maintain safety and specificity with clean shutdown.